We are hoping to duplicate a TRAP procedure  following some fixationas and
decals in some literature that one of our investigators read.  He had his
techs do the fixaing/decal/staining all we did was cut.  They used the
Sigma kit with varying results.  The investigator wants us to follow the
procedure in the literature and it states that FFPE for 24 hr fix in NBF
and then 24-48 hrs of decal in Surgipath HCL/EDTA decal.  The staining
looks different when comparing the two sections with and without tartrate,
but the pathologist says she does not believe it is staining the right
stuff.  My belief is that the decal is too harsh, and maybe there is a
better fixation method.  So now of course they want the "histotechs" to get
involved(imagine that).  Any ideas on why this stuff may not be working?

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