Re: rcc ab

we use Novocastra's aby (clone 66.4.C2) at 1:25 for 30min. RT, EnVision+ system, trypsin digestion. be aware that this aby does not seem to be stable for a long period of time in the refrig. you should aliquot and freeze it after reconstituting it. we've found it to be a pesky aby to use (and i've heard the same from others).

Carrie Kyle-Byrne, BHS, HT(ASCP)
Assoc. Research Scientist II
Molecular Target Research

Exelixis, Inc.
170 Harbor Way
P.O. Box 511
South San Francisco
CA 94083-0511 USA

Phone: (1 650) 837-8023
Fax: (1 650) 837-7240
Email: ckbyrne@exelixis.com

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