Some of the Immuno companies offer mouse on mouse detection systems.
DAKO has something called ARK (Animal Research Kit) for this purpose.
The basic concept is to react the antibody with the secondary in a test
tube then apply it to the tissue avoiding the general non specific
binding of the secondary to the tissue proteins.
Patsy Ruegg

Cathy Gorrie wrote:

> For all the immunohistologists out there.
>
> This is a pretty general question about immunohistology on mouse
> tissue. When using monoclonal antibodies, what special measures need
> to be taken, or what secondaries need to be used, in order to get
> specific staining. I can imagine if you use an anti-mouse secondary
> everything in the tissue will be targeted, or at least a lot of
> background from serum proteins, immuno cells etc.
>
> or
>
> Can you not use monoclonals on mouse tissue? I have always used other
> species of animal, or human and this has never been a problem before.
>
> or
>
> Is this not as big a problem as I imagine it could be?
>
> Thanks for your help,
>
> Cath
> --
> ---------------------------------------------------
> Cathy Gorrie
> Scientific Officer
> Neural Injury Research Unit,
> School of Medical Sciences,
> University of New South Wales
> Sydney, N.S.W. 2052
>
> Phone: 61-2-9385 2462
> Fax   : 61-2-9313 6252
> e-mail: c.gorrie@unsw.edu.au