Re: goat serum
I use protein blocks for my animal work, usually not required for my human IHC.
Cynthia Favara wrote:
> I usually do not use a protein block unless I have a problem when working up
> a new antibody.
> Most of my nonspecific background comes from in-house sticky antibodies.
> Cynthia Favara
> -----Original Message-----
> From: Richard Cartun [mailto:Rcartun@harthosp.org]
> Sent: Tuesday, March 12, 2002 6:44 AM
> To: HistoNet@pathology.swmed.edu
> Subject: Re: goat serum
> Are many people still using a protein block on their formalin-fixed,
> paraffin-embedded tissue sections for immunohistochemistry? We haven't
> used a protein block (i.e., nonimmune serum) for several years now and we
> never see nonspecific staining.
> R. Cartun
> >>> 03/05/02 03:29PM >>>
> Does anyone out there know where to purchase bulk goat serum for us to
> dilute for blocking? We are really being pushed to cut corners and have to
> stop using Dako's protein block.
> Thanks in advance,
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