Re: bone marrow decalcification
I agree that it is possible to get a very good result from microwave
decalcification. However my point was that you will not improve
your results through microwave procedures, you will only speed
them up. On the contrary , we do know that cooling a decalicifier
will improve the results.
----- Original Message -----
From: Jan Minshew
Date: Monday, March 4, 2002 2:20 pm
Subject: Re: bone marrow decalcification
> Hi Cliff,
> I'm afraid I must beg to differ. I have seen beautiful sections of
> specimens that were decalcified using microwave accelerated
> techniques and EDTA, formic acid solutions and others as well.
> EDTA is indeed slower but it can be greatly accelerated in a
> Laboratory Microwave using controlled temperatures and
> times. If time allows, its gentle action will produce specimens
> with uncompromised morphology and antigenicity, even at the
> ultrastructure level. There is an excellent chapter in Microwave
> Techniques and Protocols (ISBN 0-89603-903-X) that details the
> procedure. Organic and inorganic acids can also be used with
> excellent results. The key is being able to control the
> environment of the specimen during the procedure.
> Jan Minshew
> Histology Consulting Services, Inc.
<< Previous Message | Next Message >>