Re: antigen retrieval & Enzyme

From:rueggp

Some where along the way I was given the impression that enzyme digestion should always be done before HIER.  Can you biochemies explain this?
Patsy

Njoydobro@aol.com wrote:

Does anyone have any experience with using trypsin/pepsin following antigen retrieval?  Which ab's are people having to do this for?  & what exact steps are you taking?  Your help is appreciated.

Thanks,
Gene


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