Re: anti-Vimentin

From:Kappeler Andreas

moa-a-Vimentin, clone V9, (e.g. Dako M 0725) shows cross-reactivity with
many species, including mice (according to Dako). We have successfully used
it on several animal species, however not with mouse tissue. Be aware that
mouse-on-mouse IHC will create some problems with your secondary antibody
picking up all the endogenous mouse immunoglobulin along with the
mo-a-vimentin antibody. Commercial kits that allow to stain with mouse
antibodies on mouse tissue are available at least from Dako (ARK) and Zymed
(Histo Mouse). Hope this helps.

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

-----Ursprüngliche Nachricht-----
Gesendet: Donnerstag, 28. Februar 2002 18:20
Betreff: anti-Vimentin

> Good afternoon everybody,
> I am working on the immunohistochemical staining of anti-Vimentin on mouse
> tissue. I tried monoclonal anti-vimentin from Biogenex, Dako and Signet,I
> also used
> polyclonal anti-vimentin from Chemicon, but neither one of them worked on
> mouse tissue. I wonder anybody can give me some information on
> anti-vimentin that works on mouse tissue?
> Thank you very much!
> Huiling

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