Re: RE-Mitosis

From:Geoff McAuliffe

    I am not sure that I understand your question. You want to know if
mitosis has occured after the fact, as in, this cell divided yesterday or
several days ago? If that is your question, the answer is no. The only way
to see mitosis is to catch the cell in the act, not easy to do.
    You can see incorporation of DNA analogues such as thymidine or
bromodeoxyuridine with autoradiography or immuno, but you must give a
compound to label the cells before they start to synthesize DNA.

Lorraine Rolston wrote:

> Hi there everyone,
> I have a question here on behalf of a colleague.Is there a simple
> histology stain that will demonstrate cell mitosis within 2 weeks of it
> occuring , without using an immunohistochemical assay.Would a Methyl
> Green/ Pyronin do , or , perhaps the Feulgen reaction ? I would really
> appreciate some help with this one.Thanking you in advance.
> Cheers,Lorraine Rolston.(very new histonetter.)
> Histology Lab./Anatomy/Biomedical Sciences Division,
> University of Auckland,
> New Zealand.

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

<< Previous Message | Next Message >>