Re: Placenta Update
Re: Placenta Update
Ask your researcher to bring you a (written) copy of the journal
article with the methodology she is attempting to follow, then you
will be able to point out all the discrepancies between it and the
very clear protocol you would normally use.
I agree with what Thomas Jasper said. If the researcher can
explain to you what they intend to do with the tissue, you may be able
to compromise, or shock horror!, make helpful suggestions. Over-fixing
may possibly destroy some things (Ag's, enzymes) BUT under-fixing will
most certainly result in poor morphology and crap sections, especially
for placenta. Maybe she needs to do some more research about
alternative methods that will suit this tissue type and optimal
fixation.
Good Luck, Cath
Hi everyone,
Thank you all for responding to my
dilemma.
Here's an update on my placental problems. Not only does the
researcher have problems cutting the tissue smaller than 3 mm thick -
she also wants it in formalin a minimum amount of time before
processing (2 to 3 hours). I've explained that the
results she'll achieve under these conditions will be poor - but
she insists. Any other suggestions I may give
to her?
Louri
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