Re: Lymphoma Diagnosis
|From:||Richard Cartun (by way of Histonet)|
Don't abandon ship yet! We just submitted a manuscript showing
demonstration of clonality in 81% of 113 cases of formalin-fixed,
paraffin-embedded B-cell NHL. The secret (in our hands) was using a 96
degree waterbath (40 minutes) for antigen retrieval.
>>> Sharon Cassar 03/05/02 12:05PM >>>
Is there anyone out there who is doing immunohistochemistry on fresh lymph
node tissue for the diagnosis of lymphoma. We do immunohistochemistry on
paraffin embedded tissue and our pathologist would like to do on fresh
tissue because surface membrane immunoglobulin light-chain restriction is
not routinely demonstrated in paraffin section.
I would like to know what antibodies, antibodies source and method are being
used for frozen sections and if there is any literature about comparitve
study for the diagnosis and classification of malignant lymphomas using
frozen-section and paraffin section immunohistochemistry.
Thanks in advance
Histology Section, Pathology Dept.
St. Luke's Hospital
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