Judy are you using anti-mouse Ki67?  The anti-human Ki67 may not work on mouse
tissue.
Patsy

"Judy Trogadis (by way of Histonet)" wrote:

> Dear Histonettors
>
> Has anyone any experience in using  Ki67 antibody - marker of proliferating
> cells? We are trying to use a monoclonal antibody on frozen mouse retina
> without any success. Several of us have tried it, there must be a trick we
> are all missing.
>
> Thank you in advance for any possible suggestions.
>
> Judy
>
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 8Q
> 30 Bond St.
> Toronto, ON
> M5B 1W8
> Canada
>
> ph:  416-864-6060  x6337
> fax: 416-864-6043
> trogadisj@smh.toronto.on.ca