Re: In Situ Fluorescence
Thank you for your email suggestion. It's what I want to know. It have been
a long time since I do In Situ to detecting DNA probes, do you think you can
send me the protocol for doing it?
Many thank if you can help.
>From: "Richmond Thompson"
>To: Jasmine Piep
>Subject: Re: In Situ Fluorescence
>Date: Fri, 15 Mar 2002 09:19:58 -0500
>for the double label in situ florescence, one possibility would be to tag
>probe with digoxigenin and the other with fluorscein. then use an anti-dig
>conjugated to rhodamine (roche has them) and an anti-fluorscein conjugated
>alexa fluor 488 (which is a stronger and more stable fluorophore than is
>florescein). direct visualization of flourophore tagged probes is tough
>unless you have super strong signal. you can also do extra amplifications
>using different sets of secondary antibodies.
>Jasmine Piep wrote:
> > Hi everyone,
> > I want to do a double labeling for In Situ Fluorescence on a mouse's
> > tissues. Can anyone give me the information and the protocol to how to
> > this and where can I get a good antibodies?
> > Many thank if anyone can help.
> > Jasmine.
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>Richmond R. Thompson
>Brunswick, ME 04011
>(207) 725 - 3544
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