Re: In Situ Fluorescence

From:Jasmine Piep

Hi Richard,

Thank you for your email suggestion. It's what I want to know. It have been 
a long time since I do In Situ to detecting DNA probes, do you think you can 
send me the protocol for doing it?

Many thank if you can help.


>From: "Richmond Thompson" 
>To: Jasmine Piep 
>Subject: Re: In Situ Fluorescence
>Date: Fri, 15 Mar 2002 09:19:58 -0500
>for the double label in situ florescence, one possibility would be to tag 
>probe with digoxigenin and the other with fluorscein.  then use an anti-dig
>conjugated to rhodamine (roche has them) and an anti-fluorscein conjugated 
>alexa fluor 488 (which is a stronger and more stable fluorophore than is
>florescein).  direct visualization of flourophore tagged probes is tough
>unless you have super strong signal.  you can also do extra amplifications 
>using different sets of secondary antibodies.
>Jasmine Piep wrote:
> > Hi everyone,
> >
> > I want to do a double labeling for In Situ Fluorescence on a mouse's 
> > tissues. Can anyone give me the information and the protocol to how to 
> > this and where can I get a good antibodies?
> >
> > Many thank if anyone can help.
> >
> > Jasmine.
> >
> > _________________________________________________________________
> > Send and receive Hotmail on your mobile device:
>Richmond R. Thompson
>Assistant Professor
>Psychology Department
>Bowdoin College
>Brunswick, ME 04011
>(207) 725 - 3544

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