Re: In Situ Fluorescence

From:Richmond Thompson

for the double label in situ florescence, one possibility would be to tag one
probe with digoxigenin and the other with fluorscein.  then use an anti-dig
conjugated to rhodamine (roche has them) and an anti-fluorscein conjugated to
alexa fluor 488 (which is a stronger and more stable fluorophore than is
florescein).  direct visualization of flourophore tagged probes is tough
unless you have super strong signal.  you can also do extra amplifications by
using different sets of secondary antibodies.

Jasmine Piep wrote:

> Hi everyone,
> I want to do a double labeling for In Situ Fluorescence on a mouse's liver
> tissues. Can anyone give me the information and the protocol to how to do
> this and where can I get a good antibodies?
> Many thank if anyone can help.
> Jasmine.
> _________________________________________________________________
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Richmond R. Thompson
Assistant Professor
Psychology Department
Bowdoin College
Brunswick, ME 04011
(207) 725 - 3544

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