Re: CD15 (Leu-M1)
From: | Phyllis Davie (by way of Histonet) |
Diana,
Here is the information about what we do for CD15. Clone MMA is
really the same as Leu-M1, at least according to Becton Dickinson. I
contacted them several years ago, and got verbal assurance that they were
the same (I #025#ve been ordering it under the same catalog number for
[mumbledy-mumble] years).
CD15; clone MMA
* Mfgr/Vendor: Becton Dickinson # 347420
Becton Dickinson
Phone: 800-223-8226
Fax: 408-954-2007
* Control: spleen * Pretreatment: Steam in 10mM citrate buffer, pH
6.0(+/-0.3) for 20 minutes in vegetable steamer, followed by a 20'
cooldown. Rinse in buffer or water, then incubate slides @ RT for 10
minutes in Pronase solution (0.01% in PBS), followed by rinsing in
distilled water. Wash in buffer. * Dilution: Apply at 1:50 and incubate
for 40 minutes @RT. Wash in buffer. * Secondary: biotinylated
anti-MOUSE IgM, Vector # BA-2020, 1:400 in PBS (incubate 20 #025# @ RT) Wash in
buffer. * Detection: Elite Avidin-Biotin-Complex #020#Horseradish Peroxidase
(ABCHRP), Vector # PK-6100, made per kit instructions. Incubate 20 minutes
@RT. Wash in buffer. * Chromogen: DAB
Best of luck,
Phyllis Davie
PhenoPath Laboratories--Seattle, WA
pdavie@phenopath.com
on 3/7/02 10:36 AM, Goodwin, Diana at DGoodwin@CHSNJ.org wrote:
> Greetings, Histonetters.
>
>
> Can anyone tell me a source for CD-15 clone Leu-M1? Is this clone
> commercially available, or is it held under a patent?
>
>
> Diana Goodwin, HT
> Trenton, NJ
>
>
>
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