Re: Alcian Yellow Solution

From:Lee & Peggy Wenk

There is no more in the world. Gone. And cannot be made again.

In the last issue of the Mich. Soc. of Histotechnology newsletter,
Richard Dapson, PhD, wrote an article about the shortage of Alcian Yellow,
ANATECH's attempts to re-create it. No luck. However, just recently, ANATECH
was able to come up with a yellow dye that binds the stomach's neutral mucin
a different  mechanism. Unfortunately, I don't remember whether it is
being marketed or just about ready to be marketed. (Honest, Dick. I DID read
your article!) Maybe Dick can comment on the status of this replacement dye.

In the mean time, his advice is to make up your remaining Alcian Yellow
as a 0.2% solution, as Alcian Yellow does not dissolve any more than a 0.2%.
So, if using a 1% solution, you are wasting 0.8% that is precipitating out
to the
bottom of the solution.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Scott, Allison D" 
Sent: Monday, March 04, 2002 11:53 AM
Subject: Alcian Yellow Solution

> Hello, I hope everyone had a good weekend.  Is there anyone out there
> the alcian yellow technique for helicobacter?  We usually get our 1%
> solution from Polyscientific, but they do not carry it any more.  Does any
> one have a source for the 1% solution or the powder.  Thanks in advance.
> Allison Scott

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