----- Original Message -----
From: Sinoera Tech
To: histonet@pathology.swmed.edu
Sent: Thursday, March 14, 2002 10:50 AM
Subject: Re: Alcian yellow, Alcian Blue


Dear Mr. Bob Richmond,

Please note that we said we did not start from :"sym-dichlorodimethyl
ether". ( really this compound is carcinogen, we are not the first one to
mention this compound on histonet, if you have no this data, we can send you
in details).

Dear sir, in the recent English Suzhou is " Suzhou", not "Soochow"( very old
and no longer used anymore). Suzhou is the most developed area in China.
This spelling "Suzhou" is from standard chinese spelling, not the english
name. Just like we can not use chinese spelling to call "Tennessee" as
....etc.

Right now we don't concern the connection of Mr. Bob Richmond with Anatech.
It is not necessary for you to clarify this potential relationship if any.

We never told histonet about "BSC  address the issue of environmentally
appropriate manufacture ", we knew that BSC certified the dyes' results, not
their production process.
One thing you are right, here BSC indicates "Biological Stain Commission",
thanks for your reminding.

We must let the histofield choose Alcian Yellow, Alcian Blue or their newly
alternatives. If everybody discards Alcian Blue or Alcian Yellow, then, at
that time their really new alternatives arise.

We appreciate the success of the alternatives made by ANATECH, and we are
also pleased to find the new way to synthesize the Alcian Blue, Alcian
Yellow in bulk qty, not from "home labs", the starting materials are enough
for supplying the market needs, even tons kilos ? Their intermediates are
NOT the so-called " intermediates... impractical".

Good luck !

Kind Regards.
-
Sincerely Yours,
Minggeng Wang, Ph.D / President
SUZHOU SINOERA CHEM CO., LTD.
258 Binhe Road,
Suzhou New District,
215011 China
E-mail: billions@public1.sz.js.cn

----- Original Message -----
From: RSRICHMOND@aol.com
To: HistoNet@pathology.swmed.edu
Sent: Wednesday, March 13, 2002 9:57 PM
Subject: Re: Alcian yellow


Phil Bergin at Göteborg University in Sweden asks:

>>But is there a reason that people have stopped making Alcian Yellow? It's
a
topic that seems to come up fairly often, and I was wondering why people
have
stopped making it if its in such high demand.<<

A lot of information about Alcian yellow and Alcian blue has been exchanged
on this list. Briefly, the manufacture of these two dyes by the methods
originally used to make them had to be stopped, because some unnamed
carcinogenic synthetic intermediates could not be handled.

Dick Dapson at Anatech (a regular on Histonet, of course) worked out
alternative syntheses that did not involve these intermediates. Currently
Anatech is offering Alcian blue, and has announced the availability of
Alcian
yellow in the near future. (Note: I have no connection with Anatech.)

>From time to time Minggeng Wang, Ph.D at the Suzhou Sinoera Chem Co., Ltd.
in
China (the traditional English spelling of this place is Soochow) has
announced the availability of both dyes, most recently in a communication
yesterday. They stated that

>>Both stains are produced by safe way and environment approval, they are
not
started from the sym-dichlorodimethyl ether.<<
This is the first time I have seen the carcinogenic synthetic intermediate
named. Is this in fact the intermediate that made the manufacturing process
impractical?

Dr. Wang goes on to state:
>>Good news, we are just approved by BSC a bulk lot of Alcian Blue 8GX, lot
20020129A early this month with the help of Mr. Frank Matthew of BSC. Our
Alcian Blue 8GX is also being accepted/purchased by Merck KGaA, bulk lot.<<

Supposing that BSC is the Biological Stain Commission: can anyone on
Histonet
verify this? Did the BSC address the issue of environmentally appropriate
manufacture when they issued this approval? If they didn't, should they
have?

Bob Richmond
Samurai Pathologist
Knoxville, Tennessee USA