I've heard this more than once lately! We found that we were washing our
biopsies too long before staining - in an effort to remove the Bouin's. We
stopped that and have had no problems since. We cut on "naked water baths"
and use charged slides.

Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta


	-----Original Message-----
	From:	Chewy71874@aol.com [SMTP:Chewy71874@aol.com]
	Sent:	Wednesday, March 06, 2002 12:33 PM
	To:	HistoNet@pathology.swmed.edu
	Subject:	prostate needle biopsies washing

	We are having a problem with prostate needle biopsies for high
molecular
	weight cytokeratin.  Most are fixed in Bouin's, some in formalin,
and
	paraffin embedded.  We have tried using only DI water in the
waterbath (with
	no additives), using silanized slides, and draining the slides well
before
	drying them in a 60 degree oven for 1-2 hours.  They often are
washing during
	deparaffinizing, some during steamer pretreatment with HipH target
retrieval.
	 Is anyone else having this problem?  What have you found that works
for you?

	Ellen Yee
	Sacramento