RE: immuno on mouse tissue

From:"Philopena, Jennifer"

RE: immuno on mouse tissue
Abizar, I just used DAKO's mouse on mouse kit, called ARK (Animal Research Kit).  It worked beautifully.  But it's expensive.  Jen
-----Original Message-----
From: Abizar Lakdawalla []
Sent: Tuesday, March 05, 2002 2:08 PM
To: 'Cathy Gorrie';
Subject: RE: immuno on mouse tissue

Some vendors (including us) sell mouse on mouse kits that work quite well for using mouse monoclonal antibodies on mouse tissues.


> -----Original Message-----
> From: Cathy Gorrie []
> Sent: Monday, March 04, 2002 6:03 PM
> To:
> Subject: immuno on mouse tissue
> For all the immunohistologists out there.
> This is a pretty general question about immunohistology on mouse
> tissue. When using monoclonal antibodies, what special measures need
> to be taken, or what secondaries need to be used, in order to get
> specific staining. I can imagine if you use an anti-mouse secondary
> everything in the tissue will be targeted, or at least a lot of
> background from serum proteins, immuno cells etc.
> or
> Can you not use monoclonals on mouse tissue? I have always used other
> species of animal, or human and this has never been a problem before.
> or
> Is this not as big a problem as I imagine it could be?
> Thanks for your help,
> Cath
> --
> ---------------------------------------------------
> Cathy Gorrie
> Scientific Officer
> Neural Injury Research Unit,
> School of Medical Sciences,
> University of New South Wales
> Sydney, N.S.W. 2052
> Phone: 61-2-9385 2462
> Fax   : 61-2-9313 6252
> e-mail:


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