Thanks for all your input,

I've now got the basic concept and lots of vendors to approach.

(((Monoclonal Ab + secondary Ab) - unattached IgG) + mouse tissue) + 
visualization system = specific antigen staining with low background.

Simple really!

Cath

>
>  > ---------------------------------------------------
>>  Cathy Gorrie
>>  Scientific Officer
>>  Neural Injury Research Unit,
>>  School of Medical Sciences,
>>  University of New South Wales
>>  Sydney, N.S.W. 2052
>  >
>  > Phone: 61-2-9385 2462
>  > Fax   : 61-2-9313 6252
>  > e-mail: c.gorrie@unsw.edu.au
>  >
>  >

-- 
---------------------------------------------------
Cathy Gorrie
Scientific Officer
Neural Injury Research Unit,
School of Medical Sciences,
University of New South Wales
Sydney, N.S.W. 2052

Phone: 61-2-9385 2462
Fax   : 61-2-9313 6252
e-mail: c.gorrie@unsw.edu.au