Just to add a note to Geoff's advice.  The two enzymes are given treatment
in Vol 3 of the A.G.E. Pearse trilogy.  The tome is referenced as follows:
Stoward, P.J. and Pearse, A.G.E., Histochemistry, Vol 3 Enzyme
Histochemistry (4th Ed.), Churchill Livingstone, NY, NY, 1991.  ISBN:
0-443-02996-2.  Apparently the book is still available, but it IS pricey.
$200+. 

Regards

Fred Monson

> ----------
> From: 	Geoff McAuliffe
> Sent: 	Tuesday, March 12, 2002 8:45 AM
> To: 	HistoNet@pathology.swmed.edu
> Subject: 	Re: enzyme cytochemistry
> 
> A good text on histochemistry should do it. Try "Histological and
> Histochemical
> Methods" by John Kiernan or "Histopathologic Technique and Practical
> Histochemistry" by Lillie and Fulmer. Also "Histochemistry" by Pearse.
> 
> kwalters wrote:
> 
> > Hi everyone,
> >
> > I know no one does enzyme cytochemistry anymore, but I am looking for a
> > protocol for two enzymes, and need a source for protocols.  The enzymes
> are
> > monoamineoxidase and catechol omethyl transferase.  We are hoping to
> locate
> > these on brain tissue with an enzyme reaction.  Thanks for any help!!
> >
> > Kathy
> >
> > Kathy Walters                                //
> > Central Microscopy Research Facility        / /
> > 85 EMRB                                    / /\
> > University of Iowa                        / /\ \
> > Iowa City, Iowa 52242                    / /  \ \
> > Phone #:  (319) 335-8142                / /    \ \
> > Fax #:  (319) 384-4469                ______   ((0))
> > email: Katherine-Walters@uiowa.edu     |__|    / /
> >                                         ||    / /
> >                                      --------------
> >                                    ------------------
> 
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff@umdnj.edu
> **********************************************
> 
> 
> 
>