RE: control tissue for TUNEL

From:"Monson, Frederick C."

Why not the epiphysial plate in a long bone from a neonatal mouse (Sorry!)
that still has poorly, but partly, calcified tissue or from one of the mice
you are using.  If the mice are young, the more terminal growth zones should
provide continuous, predictable, TUNEL positivity.

Fred Monson

Frederick C. Monson, PhD   
Center for Advanced Scientific Imaging  
West Chester University  
West Chester, Pennsylvania, USA, 19383

> ----------
> From: 	Johnson, Teri
> Sent: 	Thursday, March 14, 2002 9:54 AM
> To: 	Histonet (E-mail)
> Subject: 	control tissue for TUNEL
> I'm getting ready to start working up the markers for apoptotic cells
> (TUNEL and caspase-3) in mouse tissue.  I'm wondering what tissue is best
> to use as a control.  Skin, perhaps?
> Thanks!
> Teri Johnson
> Managing Director Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri  64110

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