I process rat trachea as any other rat tissue, but when I cut the block, I
usually soak on ice because it's a bit hard to cut.
No other particular additive advice.
Good luck

Silvia
GSK Company

> -----Original Message-----
> From:	Donna Kumiski [SMTP:dkumiski@mail.mcg.edu]
> Sent:	mercoledì 13 marzo 2002 15:08
> To:	histonet@pathology.swmed.edu
> Subject:	Rat Trachea
> 
>  Anyone out there working with rat trachea? I have fixed the tissue
> overnight in 10%NBF 
> and will be processing it for paraffin sectioning. The slides will be
> processed for H&E staining and 
> Immunohistochemistry. Any info for obtaining perfect paraffin sections
> would be appreciated.
>     Thanks
> 
> Donna Kumiski
> Medical College of Georgia
> Cellular Biology and Anatomy
> 
>  dkumiski@mail.mcg.edu
> 
>