I have never found it difficult to cut and usually just process it along
with the rest of the tissue and cut it at 4 microns.  Embedding may be
tricky but you can usually see the lumen.

Gina Sennello
OSI Pharmaceuticals
Boulder CO


-----Original Message-----
From: Donna Kumiski [mailto:dkumiski@mail.mcg.edu]
Sent: Wednesday, March 13, 2002 7:08 AM
To: histonet@pathology.swmed.edu
Subject: Rat Trachea


 Anyone out there working with rat trachea? I have fixed the tissue
overnight in 10%NBF 
and will be processing it for paraffin sectioning. The slides will be
processed for H&E staining and 
Immunohistochemistry. Any info for obtaining perfect paraffin sections would
be appreciated.
    Thanks

Donna Kumiski
Medical College of Georgia
Cellular Biology and Anatomy

 dkumiski@mail.mcg.edu