Processing and cutting is not my major concern, what about immunostaining
and other stains? 

Were you performing a wide range of immunostains? 



At 11:26 AM 3/12/02 -0500, you wrote:
>We did a trial for Sakura for several months last year.  We only used
>biopsies, but saw no difference in processing or cutting.  I'm sure other
>sites did trials on routine tissue, perhaps they could give you more
>insight.
>
>Ginny Einfalt  HT/HTL(ASCP)
>Corporate Histology Manager, Virtua Health
>geinfalt@virtua.org
>
>
>-----Original Message-----
>From: Jill Songer [mailto:jtsonger@vt.edu]
>Sent: Tuesday, March 12, 2002 9:35 AM
>To: Gayle Callis; HistoNet@pathology.swmed.edu
>Subject: Re: Question about Tisue Tek VIP fixative
>
>
>Gayle,
>Funny you ask this question today. I was going to post the same question. 
>We have noticed a difference in sectioning since going to this fixative. 
>Seems tissue is not as well fixed as before. We have increased time on 
>processor.
>
>Has anyone else experienced this?
>
>
>
>At 07:45 AM 3/12/2002 -0600, Gayle Callis wrote:
>>Has anyone been using this new fixative developed by Sakura touted for
>>keeping VIP (other processors?) cleaner?? It was developed to prevent
>>tubing from clogging and eliminate the need for water rinses after cleaning
>>cycle.
>>
>>If so, questions:
>>
>>A formalin fixative buffered in some proprietary way - how does is compare
>>to routine NBF?
>>
>>Have you noticed any changes in immunohistochemistry, routine staining, and
>>other special stains
>>
>>
>>Gayle Callis
>>MT,HT,HTL(ASCP)
>>Histopathology Supervisor
>>Veterinary Molecular Biology - Marsh Lab
>>Montana State University - Bozeman
>>19th and Lincoln St
>>Bozeman MT 59717-3610
>>
>>406 994-6367
>>406 994-4303 (FAX)
>>
>
>
>**************************************************************************
>Jill Songer HT (ASCP)
>Supervisor, Anatomic Pathology
>Veterinary Medical Teaching Hospital
>Virginia Tech
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)