RE: Question about Tisue Tek VIP fixative

From:Gayle Callis

Processing and cutting is not my major concern, what about immunostaining
and other stains? 

Were you performing a wide range of immunostains? 

At 11:26 AM 3/12/02 -0500, you wrote:
>We did a trial for Sakura for several months last year.  We only used
>biopsies, but saw no difference in processing or cutting.  I'm sure other
>sites did trials on routine tissue, perhaps they could give you more
>Ginny Einfalt  HT/HTL(ASCP)
>Corporate Histology Manager, Virtua Health
>-----Original Message-----
>From: Jill Songer []
>Sent: Tuesday, March 12, 2002 9:35 AM
>To: Gayle Callis;
>Subject: Re: Question about Tisue Tek VIP fixative
>Funny you ask this question today. I was going to post the same question. 
>We have noticed a difference in sectioning since going to this fixative. 
>Seems tissue is not as well fixed as before. We have increased time on 
>Has anyone else experienced this?
>At 07:45 AM 3/12/2002 -0600, Gayle Callis wrote:
>>Has anyone been using this new fixative developed by Sakura touted for
>>keeping VIP (other processors?) cleaner?? It was developed to prevent
>>tubing from clogging and eliminate the need for water rinses after cleaning
>>If so, questions:
>>A formalin fixative buffered in some proprietary way - how does is compare
>>to routine NBF?
>>Have you noticed any changes in immunohistochemistry, routine staining, and
>>other special stains
>>Gayle Callis
>>Histopathology Supervisor
>>Veterinary Molecular Biology - Marsh Lab
>>Montana State University - Bozeman
>>19th and Lincoln St
>>Bozeman MT 59717-3610
>>406 994-6367
>>406 994-4303 (FAX)
>Jill Songer HT (ASCP)
>Supervisor, Anatomic Pathology
>Veterinary Medical Teaching Hospital
>Virginia Tech
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

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