RE: Placenta Update

From:"Haines, Ashley N."

Hey All,
Perhaps we could move on?  Remember that there are PIs on this listeserve and that there are PIs who do their own histology, and well I might add.  So unless we have some technical suggestions for Louri, I think you all have given her the best advice on how to deal with the situation: Communicate with the PI, sit down and talk about how to achieve the best results.  I'm sure you can work it out.
-----Original Message-----
From: rueggp []
Sent: Monday, March 04, 2002 4:45 PM
To: PMarcum
Cc: Geoff McAuliffe; Louri Caldwell;
Subject: Re: Placenta Update

unfortunately it is true.  the PI will never admit that bad sections are related to anything they did.  the problem is that they do not know about processing, microtomy or staining tissue.  all they know is some gross anatomy and what they think they want to see under the microscope.  they usually not only don't know how to get good samples under the microscope, they also are not interested in knowing.
patsy ruegg

PMarcum wrote:

 I am in way answering this for Louri without her permission.  Unfortunately it doesn't work that way.  She will be blamed for lousy work unless her boss or someone higher up who knows what they are doing steps in and gets the PI under control.  I have been through this myself several times with research material.  Pam Marcum
-----Original Message-----
From: Geoff McAuliffe [mailto:mcauliff@UMDNJ.EDU]
Sent: Monday, March 04, 2002 10:33 AM
To: Louri Caldwell
Subject: Re: Placenta Update
Give her exactly what she wants, she is obviously an "expert". Then she can defend her lousy results when she shows the slides at a meeting or seminar.

Louri Caldwell wrote:

Hi everyone, Thank you all for responding to my dilemma.
Here's an update on my placental problems.  Not only does the researcher have problems cutting the tissue smaller than 3 mm thick - she also wants it in formalin a minimum amount of time before processing (2 to 3 hours).    I've explained that the results she'll achieve under these conditions will be poor - but she insists.  Any other suggestions I may give to her? Louri
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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