RE: Placenta Update
Re: Placenta Update
I
really like this suggested approach. In ten years of working with graduate
students and various non pathologists for research projects I found very
few that were not willing to listen and try different methods when the
methods were explained with a sound scientific basis. I have found similar
results working with corporate scientists for the past 13 years. Most of these
scientists are not trained in histotechnology or pathology. I've always tried to
convey to them that they are the experts in their area, but we are the experts
in our area and we'll get a much better result working together. That said there
are the few exceptions that prove the rule. I've found that most of those
individuals can be won over by a technique I've always called, "doing a
Sheehan." Very early in my career I attended a roundtable discussion chaired by
Dezna Sheehan. This topic came up in the course of discussion. My memory is
somewhat hazy about the specifics, but either Dezna or one of the people working
with her told the following story. One day Dezna was in the grossing room with a
resident and saw him about to put an adrenal gland into formalin. She suggested
that he should put some of the tissue into Orth's fluid since the differential
diagnosis was pheochromocytoma and formalin would dissolve the characteristic
granules necessary for diagnosis. The resident assured her in no uncertain terms
that he knew what he was doing and didn't need her advice. As soon as he left
the grossing area she went back to the specimen container and retrieved a small
portion of tissue for Orth's fixation, processing, and staining. Dezna routinely
attended slide reviews and was present when the case was reviewed. Dr.
Enterline asked the resident where were the Orth's fixed slides since they were
essential for the diagnosis. Just as the resident was about to admit to his
error, Dezna pulled a slide try out from her papers and said, "Here they are
sir. The resident loaned them to me for review and I forgot to return them." The
resident developed a smile that probably wrapped almost round his entire head
and they were fast friends and colleagues for the rest of his tenure and
throughout his professional career. Now I'm not sure the story happened exactly
that way, but I have been able to do "favors" such as this for residents and PIs
who just didn't want to listen and afterward most of them have been great to
work with after seeing the results. For that very small minority that even this
doesn't work with - for them I think you do have to have an authority figure to
act as a go-between or try to work with them as little as possible. I've always
found this method to work better than pointy sticks, verbal barbs, or in any
other way trying to belittle the person involved. Best of
luck.
John E. Tarpley 5-1-A
Associate Scientist
Amgen
Inc.
One Amgen Center Drive
Thousand Oaks, CA 91320
These Opinions are
my own and not necessarily those of my employer.
Ask your researcher to bring you a (written) copy of the journal article
with the methodology she is attempting to follow, then you will be able to
point out all the discrepancies between it and the very clear protocol you
would normally use.
I agree with what Thomas Jasper said. If the researcher can explain to
you what they intend to do with the tissue, you may be able to compromise, or
shock horror!, make helpful suggestions. Over-fixing may possibly destroy some
things (Ag's, enzymes) BUT under-fixing will most certainly result in poor
morphology and crap sections, especially for placenta. Maybe she needs to do
some more research about alternative methods that will suit this tissue type
and optimal fixation.
Good Luck, Cath
Hi everyone,
Thank you all for responding to my
dilemma.
Here's an update on my placental
problems. Not only does the researcher have problems cutting the
tissue smaller than 3 mm thick - she also wants it in formalin a minimum
amount of time before processing (2 to 3 hours). I've
explained that the results she'll achieve under these conditions will be
poor - but she insists. Any other suggestions I may give
to her?
Louri
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