RE: Placenta

From:"Charles W. Scouten, Ph.D."

There are a number of instruments to help with this, varying in price and speed of operation.  
A manual tissue chopper will cut sections as thin as 250 microns (0.25mm) very rapidly (very little prep work) if just one or two are needed.  Here is a link to such an instrument:
An automated version of this is the McIlwain Tissue Chopper:

You chop, advance the tissue a measured distance, and chop again.  250 microns will work.
You can get as thin as 40 microns fresh tissue with a Vibratome(tm).
The manual tissue chopper is probably faster to use than the hand slicing being done now.
Charles W.  Scouten, Ph.D.
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300  
FAX  314 522 0277
-----Original Message-----
From: Louri Caldwell [] 
Sent: Friday, March 01, 2002 9:26 AM
Subject: Placenta
I'm sorry for the double post - I hit the "send" button too soon.
Hi Everyone,
I have a researcher who is working with placenta.  The problem: she wants to show all the layers, is cutting the tissue 5 mm thick and cramming it into a cassette, and is not satisfied with the results.  I have explained to her that it will be necessary to cut the tissue thinner before placing it into the cassette to achieve adequate processing, but she states this is too difficult.  
Does anyone have any suggestions as to an "easy" way to gross placenta into thinner sections?
Any assistance you may provide is greatly appreciated.
Thank you,
Louri Caldwell

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