RE: Ki 67 antibody

From:Robert Geske

Dako has a relatively new antibody that is rat anti mouse Ki-67.  it works
like a charm on mouse tissue that have been formalin fixed (ours were 18 to
24 hours) and routinly paraffin processed.


-----Original Message-----
From: rueggp
To: Judy Trogadis (by way of Histonet)
Sent: 3/12/02 10:53 AM
Subject: Re: Ki 67 antibody

Judy are you using anti-mouse Ki67?  The anti-human Ki67 may not work on

"Judy Trogadis (by way of Histonet)" wrote:

> Dear Histonettors
> Has anyone any experience in using  Ki67 antibody - marker of
> cells? We are trying to use a monoclonal antibody on frozen mouse
> without any success. Several of us have tried it, there must be a
trick we
> are all missing.
> Thank you in advance for any possible suggestions.
> Judy
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 8Q
> 30 Bond St.
> Toronto, ON
> M5B 1W8
> Canada
> ph:  416-864-6060  x6337
> fax: 416-864-6043

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