Evans Blue has been used as a vital dye for years to investigate vascular
injuries which lead to extravasation of blood and plasma proteins,
specifically abumenoids.

Evans blue combines with albumen stoichimetrically and the albumen-Evans
Blue complex is fluorescent.

It has been applied in the following ways:

		1.	injected i.v. as dye only
		2.	injected i.v. as dye-albumen complex

In the first method, the dye immediately combines with blood albumen and as
long as the injection contained less than an excess, all of the dye is
supposed to react with free albumen.

Use "Evans Blue" at PubMed (http://www.ncbi.nlm.nih.gov/) [PubMed is a
choice in the "Select" option box].

Regards,

Fred Monson

Frederick C. Monson, PhD   
Center for Advanced Scientific Imaging  
West Chester University  
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson@wcupa.edu


> ----------
> From: 	Kimberly L Merriam
> Sent: 	Tuesday, March 12, 2002 2:27 PM
> To: 	Histonet
> Subject: 	Evan's Blue
> 
> <>
> Does anyone know what this is used for?  Is it a vital dye?
> I can't find any information on this in any of my reference
> books.
> 
> Thanks,
> 
> Kim Merriam
> TKT
> Cambridge, MA
> 
>