Decalcification of vertebrae + spinal cord

From:"A. Erickson"

Good morning,
I have been asked to prepare sections of vertebrae with spinal cord still
in position, ultimately for an atlas.  Major concerns are decalcification
without destroying the cord, maintaining the proper relationship of cord
elements and bone elements, and processing with minimal shrinkage.  Any
information which might help with this project (ideas, suggestions,
warnings, protocols...) is greatly appreciated.  Andra Erickson, Research
Technologist, University of Washington RPRC, Neuro Division.





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