Anti-keratin cocktail to stain all epithelia
Hello!
I'm trying to build an anti-keratin cocktail capable of
staining all bovine epithelia. To keep the costs down,
I'd of course like to buy as few separate antibodies as
possible. What kind of wide-spectrum cocktails are you
using?
I currently have the following antibodies, which together
stain almost all epithelia, but not quite all of them:
AE1/AE3
Lu-5
34bE12
The tissues needing improvement are mainly parts of thymus,
some of the renal tubuli, and to some extent liver and
thyroid gland. The cytokeratin expression patterns might be
a bit different in the cow and in human, though. So I'd
just like to get a cocktail staining as many cytokeratin
types as possible.
I know there was some discussion of keratin cocktails
here a couple of years ago but that wasn't really helpful
for me.
Thanks.
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Mikael Niku URL: www.helsinki.fi/~mniku/
University of Helsinki Dept. Basic Veterinary Sciences
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