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<P>Has anyone done vascular perfusion fixation with Bouins' for IHC in rodent<BR>brains?<BR><BR>If so<BR>1.)how much/long to prefuse?<BR>2.) post-prefusion fixation , if any? (time and temperature)<BR>3.) Wash - a) in what?<BR> b) how long and what temp?<BR><BR>any advice is appreciated.<BR><BR>Peter<BR></P></DIV><br clear=all><hr>Get your FREE download of MSN Explorer at <a href="http://explorer.msn.com">http://explorer.msn.com</a><br></p></html>