rat on mouse immunos

From:Rita Angel <angelrj@email.uc.edu>

Hi histonetters,

I have an immuno question for the animal research people out there.  I have
a mac-3 antibody which is rat anti-mouse.  I will be staining mouse tissue.
  What kind of blocks do you use to block endogenous staining?  Our normal
block to use is 0.5ml of 2% mouse serum in 25mls of secondary.  I rotate
this for 1 hr. to allow binding and then use at the normal time (10 min.)
Does anyone else have any other procedure that they use?  This did not work
the last time I used it.

Thank you for your input,

Rita Angel

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