rat on mouse immunos
|From:||Rita Angel <email@example.com>|
I have an immuno question for the animal research people out there. I have
a mac-3 antibody which is rat anti-mouse. I will be staining mouse tissue.
What kind of blocks do you use to block endogenous staining? Our normal
block to use is 0.5ml of 2% mouse serum in 25mls of secondary. I rotate
this for 1 hr. to allow binding and then use at the normal time (10 min.)
Does anyone else have any other procedure that they use? This did not work
the last time I used it.
Thank you for your input,
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