labeling of bone

From:Vicki Kalscheur <>

     George Spurr-
     Acid methods of decalcification will destroy all fluorochrome labels 
     due to liberation of calcium from its ionic state through ionic 
     exchange, rendering a soluble calcium salt in the matrix. I am not 
     aware of any advances in paraffin processing that will preserve bone 
     Vicki Kalscheur

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