There are a couple ways to fix tet labeled bone.  Either fix initially in
10% neutral buffered formalin or go right into 70% ethanol.  I believe some
techs even use a product called Pen-Fix.  I co-authored a paper that may be
helpful to your study:  Sanderson-Mayton, C, Clark, N., Bachus, K. N.:  A
new look at formalin fixation for fluorochrome labeled bone.  J of
Histotechnol, 21(4)299-302,1998.

Good luck with your study and I hope this helps.
Regards,
Cathy


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GLP Compliant Laboratory

Cathy A. Mayton
Project Director
Wasatch Histo Consultants, Inc.
www.wasatchhisto.com