decalcification and cryostats
From: | jerry hu <whojerry@yahoo.com> |
Hello Everyone,
I have been through the histonet archives and found
many recipies for bone decalcification, and have
looked at some books on this topic. However, I have
also noticed that bone is generally processed through
paraffin, though what I have avaliable is a cryostat -
are there major differences that I should be aware of?
What I plan to do is fix my specimen (3 mm thick) for
24 hours in formalin, then decalcify in buffered
formic acid (pH ~2), and do endpoint determination
with ammonium oxalate. Then I would like to
freeze-embed the decalcified specimen in freeze
embedding compound and section on a cryotome. Does
the freeze embedding medium need to infiltrate the
specimen (as is the case with paraffin processing) to
cut well?
What I would like to do is to find the thickness of
the cartilage above the bone with H/E. Any comments
or experience on this will be most appreciated. I
would be *really happy* if there is a way to get
around
decalcifying the bone. Thanks!
Jerry Hu
Rice University
Houston, Texas, USA
PS sorry about the generic yahoo address - my school
e-mail system does not allow me to post to Histonet -
kinda like the problem John Kiernan is facing.
__________________________________________________
Do You Yahoo!?
Get email at your own domain with Yahoo! Mail.
http://personal.mail.yahoo.com/
<< Previous Message | Next Message >>