Me again.
A few days ago I was asking about Timm's stain for neurodegeneration. Thank 
you Geoff McAuliffe, John Kiernan, and others who sent advice. In lieu of 
doing the Marchi method "we" have decided to do the Luxol Fast Blue - Oil 
Red O Stain. :-)
Everybody mentions LFB-ORO in their discussion but I don't have a real 
procedure. What I need to know is this, do you do the LFB and then the Oil 
Red O? I'm figuring this is how to go about it. And, does it matter if you 
use Oil Red made up in Isopropyl Alcohol or Prop. Glycol?
Another method talks of Neutral Red to include Nissl. I have Neutral Red 
Iodide. Is this what I want to use? If not I'll need to get plain old 
Neutral Red.
Thanks, thanks in advance. What would I do without histonet?
Andi
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html