> kwalters wrote:
>
> > Oh wise ones,
> >
> > Does anyone have a stain for detecting zinc ions in brain tissue, either
> > paraffin or frozens?
> >
> > Thanks in advance,
> > Kathy
> >
> > Kathy Walters                                //
> > Central Microscopy Research Facility        / /
> > 85 EMRB                                    / /\
> > University of Iowa                        / /\ \
> > Iowa City, Iowa 52242                    / /  \ \
> > Phone #:  (319) 335-8142                / /    \ \
> > Fax #:  (319) 384-4469                ______   ((0))
> > email: Katherine-Walters@uiowa.edu     |__|    / /
> >                                         ||    / /
> >
>
> Kathy:
>
>     Timm's silver sulfide method will show zinc, a colleague used to use it
> in mouse CNS. Requires special fixation if I remember correctly. Also,
> dimethylglyoxime will react with zinc to give a cherry red color (I think).
> I don't know if either of these two methods is specific for zinc.
>

Kathy:

    MY mistake! Dithizone will show zinc, dimethylglyoxime is for nickel. Both
dithizone and Timm's are in John Kiernan's book, as well as some other methods
for zinc.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************