kwalters wrote:

> Oh wise ones,
>
> Does anyone have a stain for detecting zinc ions in brain tissue, either
> paraffin or frozens?
>
> Thanks in advance,
> Kathy
>
> Kathy Walters                                //
> Central Microscopy Research Facility        / /
> 85 EMRB                                    / /\
> University of Iowa                        / /\ \
> Iowa City, Iowa 52242                    / /  \ \
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> email: Katherine-Walters@uiowa.edu     |__|    / /
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>

Kathy:

    Timm's silver sulfide method will show zinc, a colleague used to use it
in mouse CNS. Requires special fixation if I remember correctly. Also,
dimethylglyoxime will react with zinc to give a cherry red color (I think).
I don't know if either of these two methods is specific for zinc.

Geoff
--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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