Re: immunodiluent survey results.

From:Dana Settembre <settembr@UMDNJ.EDU>

On Tue, 27 Mar 2001, Aidan Schurr wrote:

> Hi all
> 
> Survey results as follows:
> 
> Diluent                       # responses
> 0.1% BSA in PBS:            3
> 1% BSA in PBS:               4
>    + tween or triton-X:        3
>    + Azide                          2
> Commercial                       8
> PBS                                  1
> 
> So it seems the majority of us use commercial diluent, followed closely by BSA in PBS (of various concentrations - I find myself wondering if the dilution is important at all!)
> 
> Interesting!
> 
> Aidan
> New Zealand
> 
> 
> 
> __
> 
> aidan schurr  b.m.l.sc
> section head, histology
> hutt valley health
> lower hutt
> new zealand
> 
> aidan.schurr@hvh.co.nz
> ++64 4 570 9173
> 
> 
> 
The diluent probably is important but there are obviously my ways that
work.  I myself have tried many diluents in the past ten years and they
all work.
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey
USA




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