Re: immunodiluent survey results.
From: | Dana Settembre <settembr@UMDNJ.EDU> |
On Tue, 27 Mar 2001, Aidan Schurr wrote:
> Hi all
>
> Survey results as follows:
>
> Diluent # responses
> 0.1% BSA in PBS: 3
> 1% BSA in PBS: 4
> + tween or triton-X: 3
> + Azide 2
> Commercial 8
> PBS 1
>
> So it seems the majority of us use commercial diluent, followed closely by BSA in PBS (of various concentrations - I find myself wondering if the dilution is important at all!)
>
> Interesting!
>
> Aidan
> New Zealand
>
>
>
> __
>
> aidan schurr b.m.l.sc
> section head, histology
> hutt valley health
> lower hutt
> new zealand
>
> aidan.schurr@hvh.co.nz
> ++64 4 570 9173
>
>
>
The diluent probably is important but there are obviously my ways that
work. I myself have tried many diluents in the past ten years and they
all work.
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark, New Jersey
USA
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