I think what she meant to say was incompatible or unstable instead of
immiscible. Immiscible means they do not mix together such as water and
oil do not mix together. I have had this same problem with certain
vendors antibody diluents also. I have not had this problem since I
started making my own antibody diluent.

Marsha Price

On Thu, 15 Mar 2001 18:32:42 -0500 Katri Tuomala <katri@istar.ca> writes:
> Penny,
> I concur with Dana about different manufacturer's antibody diluents. 
> Some antibodies of one company may not be stable with another's 
> diluent. I had a problem with an actin antibody, that would break 
> down in a few days and give a lot of background staining until I 
> went back to using the same company's diluent.
> This problem may not arise, if you only dilute what you will use 
> that day, but I want to be able to store the diluted antibody at 
> least a few weeks, if not months.
> Katri
> 
> Katri Tuomala
> Hamilton, Ontario, Canada
> 
> Penelope Marr wrote:
> 
> > Dana,
> > What do you mean that diluents are not miscible???  They are all 
> aqueous
> > solutions of some sort - buffered, with a protein carrier, sodium 
> azide and
> > some have a blocking agent.  The detection systems are all made in 
> similar
> > solutions so how can any of these immiscible?
> >
> > Penny
> >
> > Penelope Marr
> > Immunohistochemist
> > S.E.A.L.S.
> > St George Hospital
> > Gray St
> > Kogarah  NSW  2218
> > Australia
> >
> > > -----Original Message-----
> > > From: DDittus787@aol.com [SMTP:DDittus787@aol.com]
> > > Sent: Tuesday, March 13, 2001 2:49 PM
> > > To:   Aidan.Schurr@hvh.co.nz; histonet@pathology.swmed.edu
> > > Subject:      Re: immunodiluent
> > >
> > > Dear Aidan
> > > Over a very long period of time I have found diluents that are 
> not
> > > miscible ,
> > > so to speak with certain companies detections, however if you 
> use their
> > > diluent you get the expected result, so we use our 3 main 
> suppliers of
> > > antibodies diluents.
> > >
> > >
> > >  Dana
> > 
>
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