Re: Silver enhancement for immunogold

From:"J. A. Kiernan" <>

On Tue, 27 Mar 2001, Cristiano Rumio wrote:

> Some of you have idea how to prepare the silver enhancement solution for 
> immunogold? I know the special kits exist, but they are to expensive.

There are various solutions that can be used. Here are a few refs.
The paper by Danscher et al is particularly thorough. Pohl &
Stierhof is for EM (preventing extraction of silver by osmication).

Holgate, C. S., Jackson, P., Cowen, P. N. and Bird,
C. C. 1983. Immunogold-silver staining: a new method of immunostaining
with enhanced sensitivity. J. Histochem. Cytochem. 31: 938-944.

Smiley, J. F. and Goldman-Racic, P. S. 1993. Silver-enhanced
diaminobenzidine-sulfide (SEDS): a technique for high resolution
immunoelectron microscopy demonstrated with monoamine immunoreactivity in
monkey cerebral cortex and caudate. J. Histochem. Cytochem. 41: 1393-1404.

Danscher, G., Hacker, G. W., Grimelius, L. and Norgaard,
J. O. R. 1993. Autometallographic silver amplification of colloidal
gold. J. Histotechnol. 16: 201-207.

Pohl, K. and Stierhof, Y. D. 1998. Action of gold chloride ("gold
toning") on silver-enhanced 1 nm gold
markers. Microsc. Res. Tech. 42: 59-65.

Newman, G. R. and Jasani, B. 1998. Silver development in microscopy and
bioanalysis: a new versatile formulation for modern
needs. Histochem. J. 30: 635-645.

Fujimori, O. 1999. Protein A-gold/silver staining method: A review of a
highly sensitive technique for light microscopic
immunohistochemistry. Appl. Immunohistochem. Mol. Morphol. 7: 280-288.
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London,  Canada   N6A 5C1

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