Tarik,

I hope you have a good atlas. I use "The Rat Brain in Stereotaxic
Coordinates" by Paxinos and Watson. I would not be able to find my way
around a rodent brain without it. The MFB looks fairly obvious, but perhaps
you could try to do a combination stain of Cresyl and Luxol Fast Blue.

Good luck-

Lorraine Gibbs
University of Washington
Physiology & biophysics
-----Original Message-----
From: Khaznadar, Tarik <T.Khaznadar@vernalis.com>
To: 'Histonet@pathology.swmed.edu' <Histonet@pathology.swmed.edu>
Date: Thursday, March 22, 2001 6:21 AM
Subject: Neuro-histology query


>
>
>Hi Dear Histoneters,
>
>I got a question here. I am trying to perform some Nissl staining in order
>to visualise neurones in the rodent MFB (medial forebrain bundle,
structures
>that projects dopaminergic outputs towards the ventral striatum). However
>the MFB is tiny and to my knowledge there is no landmark structure close
>enough to be able to identify it with certainty.
>Has anyone got clues, previous experience, alternative straining
procedures?
>
>
>Any help will be greatly appreciated
>
>
>Kind Regards
>
>>
>>
>>Tarik Khaznadar
>>Vernalis Research Limited
>>Reading
>>U.K
>
>