You can get fresh lacmoid (cat. no. 27,472-0, $23.60 for 10 g.) from Aldrich
(1-800-558-9160).
Lacmoid is a bis-resorcinyl oxazine.  Orcein is a mixture of mono-resorcinyl
oxazines.

        Allen A. Smith
        Barry University
        Miami Shores, Florida

----- Original Message -----
From: Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au>
To: <histoNet@Pathology.swmed.edu>
Sent: Thursday, March 15, 2001 12:05 AM
Subject: LACMOID RE: OOCYTES - HELP!!!!


>
> Dear Histonetters,
>
> Can anyone give me any suggestions on how to prepare a lacmoid solution
for ooctye staining?
>
> We found it very difficult to get lacmoid crystals, and the batch we
finally got our hands on is very old.  I needed to prepare a 1% lacmoid
solution and placed 1g of lacmoid crystals in 45% acetic acid and left it on
a stirrer/hot plate for 2 hours at a moderate heat.  When I filtered it
there was still a large amount of crystal undissolved (about half), yet a
reasonable amount of colour in the solution.  The undissolved crystal was
more grey in colour than they grey/burgundy of the original crystal.
>
> Does anyone know if this is an appropriate method of preparation?  I can't
believe my solution is a 1% solution if there is undissolved crystal?
>
>
> BRUCE ABALOZ
> HISTOLOGIST
> DEPARTMENT of ZOOLOGY            *  PH:    +61 3 83446282
> The UNIVERSITY of MELBOURNE        *  FAX:   +61 3 83447909
> Parkville, Victoria.3010           *  EMAIL:
b.abaloz@zoology.unimelb.edu.au
> AUSTRALIA.
>                     DANCE, LIKE NO - ONE'S WATCHING!!
>           YOU ARE A SPIRITUAL BEING HAVING A HUMAN EXPERIENCE!!
>
>
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