Re: LACMOID RE: OOCYTES - HELP!!!!
|From:||"Dr. Allen A. Smith" <firstname.lastname@example.org>|
You can get fresh lacmoid (cat. no. 27,472-0, $23.60 for 10 g.) from Aldrich
Lacmoid is a bis-resorcinyl oxazine. Orcein is a mixture of mono-resorcinyl
Allen A. Smith
Miami Shores, Florida
----- Original Message -----
From: Bruce Abaloz <email@example.com>
Sent: Thursday, March 15, 2001 12:05 AM
Subject: LACMOID RE: OOCYTES - HELP!!!!
> Dear Histonetters,
> Can anyone give me any suggestions on how to prepare a lacmoid solution
for ooctye staining?
> We found it very difficult to get lacmoid crystals, and the batch we
finally got our hands on is very old. I needed to prepare a 1% lacmoid
solution and placed 1g of lacmoid crystals in 45% acetic acid and left it on
a stirrer/hot plate for 2 hours at a moderate heat. When I filtered it
there was still a large amount of crystal undissolved (about half), yet a
reasonable amount of colour in the solution. The undissolved crystal was
more grey in colour than they grey/burgundy of the original crystal.
> Does anyone know if this is an appropriate method of preparation? I can't
believe my solution is a 1% solution if there is undissolved crystal?
> BRUCE ABALOZ
> DEPARTMENT of ZOOLOGY * PH: +61 3 83446282
> The UNIVERSITY of MELBOURNE * FAX: +61 3 83447909
> Parkville, Victoria.3010 * EMAIL:
> DANCE, LIKE NO - ONE'S WATCHING!!
> YOU ARE A SPIRITUAL BEING HAVING A HUMAN EXPERIENCE!!
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