Re: LACMOID RE: OOCYTES - HELP!!!!
|From:||"Dr. Allen A. Smith" <email@example.com>|
You can get fresh lacmoid (cat. no. 27,472-0, $23.60 for 10 g.) from Aldrich
Lacmoid is a bis-resorcinyl oxazine. Orcein is a mixture of mono-resorcinyl
Allen A. Smith
Miami Shores, Florida
----- Original Message -----
From: Bruce Abaloz <firstname.lastname@example.org>
Sent: Thursday, March 15, 2001 12:05 AM
Subject: LACMOID RE: OOCYTES - HELP!!!!
> Dear Histonetters,
> Can anyone give me any suggestions on how to prepare a lacmoid solution
for ooctye staining?
> We found it very difficult to get lacmoid crystals, and the batch we
finally got our hands on is very old. I needed to prepare a 1% lacmoid
solution and placed 1g of lacmoid crystals in 45% acetic acid and left it on
a stirrer/hot plate for 2 hours at a moderate heat. When I filtered it
there was still a large amount of crystal undissolved (about half), yet a
reasonable amount of colour in the solution. The undissolved crystal was
more grey in colour than they grey/burgundy of the original crystal.
> Does anyone know if this is an appropriate method of preparation? I can't
believe my solution is a 1% solution if there is undissolved crystal?
> BRUCE ABALOZ
> DEPARTMENT of ZOOLOGY * PH: +61 3 83446282
> The UNIVERSITY of MELBOURNE * FAX: +61 3 83447909
> Parkville, Victoria.3010 * EMAIL:
> DANCE, LIKE NO - ONE'S WATCHING!!
> YOU ARE A SPIRITUAL BEING HAVING A HUMAN EXPERIENCE!!
This message is confidential;its contents do not constitute a commitment by
The University Of Melbourne except where provided for in a written agreement
between you and The University Of Melbourne. Any unauthorised disclosure,
use or dissemination, either whole or partial, is prohibited. If you are not
the intended recipient of the message, please notify the sender immediately.
<< Previous Message | Next Message >>