Re: IHC counterstains

From:Geoff McAuliffe <mcauliff@UMDNJ.EDU>

richard hazelton wrote:

> howdy all
> Why is there such an obsession to use methyl green as a counterstain when
> thionin is a much more beautiful, and user friendly stain to use ?
> It not only stains nuclei and cytoplasm, but also contrasts equally well
> with DAB or red chromogens.
> Try it and you'll never go back to methyl green or haematoxylin for that
> matter.

Toluidine Blue is my favorite for counterstaining IHC, especially after
Vector's Intensifier for DAB (gold cells with blue nuclei). Tol blue is very
soluble in alcohol so dehydration with actone is required.

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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