Re: Fwd: (methyl benzoate)
From: | "J. A. Kiernan" <jkiernan@uwo.ca> |
On Fri, 30 Mar 2001, Dr. Ian Montgomery. wrote:
> Using acetone at -80C you are freeze substituting the tissue.
> Depending on size of tissue sample this can take many days - remember,
> small is beautiful. If possible use a rotator in the freezer or frequently
> agitate the tissue. Warm up slowly and evenly, probably over a day. Do not
> use any solvents with water, your tissue is dehydrated so why hydrate
> again. Methyl benzoate, mmmmm, wonder why, it is a clearing agent, not my
> first choice, but can be used to make tissue less brittle. Xylene is used
> here as a linking agent between methyl benzoate and wax.
I think Ian says it all here. The one thing against methyl
benzoate is its smell. Methyl salicylate (oil of wintergreen)
is much nicer. The only advantage of methyl benzoate that I know
is that it has a higher refractive index than any other clearing
agent, but this isn't an issue unless you want transparent
whole-mounts.
----------------------------------------
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan
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