Dr. Montgomery,

How are you coming to your figures ---are you assuming that the other
preparations are made in-house?  I get the same figures if I calculate
them this way.  Since I somehow missed the original message on this
survey, i don't know if it was specified whether the BSA/PBS diluents
are in-house or commercially prepared. Aren't there commercial
preparations of PBS with BSA?  Couldn't these also be included is
commercial as well as in-house prep???  that would certainly change the
results, eh?  *g*

Just curious.

Connie McManus


"Dr. Ian Montgomery." wrote:
> 
> Aiden,
>         Have to disagree with your interpretation. When you do the
> sums 38.1% use a commercial diluent, while the sensible 61.9% make
> their own.
> Ian.
> 
> > Date: Tue, 27 Mar 2001 15:23:16 +1200
> > From: Aidan Schurr <Aidan.Schurr@hvh.co.nz>
> > Subject: immunodiluent survey results.
> > To: histonet@pathology.swmed.edu
> >
> > Hi all
> >
> > Survey results as follows:
> >
> > Diluent                       # responses
> > 0.1% BSA in PBS:            3
> > 1% BSA in PBS:               4
> >    + tween or triton-X:        3
> >    + Azide                          2
> > Commercial                       8
> > PBS                                  1
> >
> > So it seems the majority of us use commercial diluent, followed
> > closely by BSA in PBS (of various concentrations - I find myself
> > wondering if the dilution is important at all!)
> >
> > Interesting!
> >
> > Aidan
> > New Zealand
> >
> >
> >
> > __
> >
> > aidan schurr  b.m.l.sc
> > section head, histology
> > hutt valley health
> > lower hutt
> > new zealand
> >
> > aidan.schurr@hvh.co.nz
> > ++64 4 570 9173
> 
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ.
> Tel: 0141 339 8855.  Extn:6602.
> Fax: 0141 330 2923
> e-mail: ian.montgomery@bio.gla.ac.uk

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