Re: Destain haematoxylin, not DAB
|From:||Amos & Theresa <email@example.com>|
Acid alcohol (0.1& HCl in 70% EtOH) will take out the hematoxylin nicely.
It's hard to remove DAB even if you want to.
Tracey Gunn wrote:
> A client wishes to remove haematoxylin from an immuno slide stained with
> DAB. She has left the slide in haematoxylin too long, and now the DAB is
> extremely hard to see. Is there any way we can remove the haematoxylin
> without removing the DAB?
> Tracey Gunn,
> Histology Service Unit,
> Otago University,
> NEW ZEALAND.
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