Re: Daily Digest
| From: | Maura Farquharson <M.Farquharson@udcf.gla.ac.uk> |
At 00:26 17/03/01 -0500, you wrote:
>Date: 16 Mar 2001 13:09:09 -0600
>From: "Hagerty, Marjorie A." <mhagerty@emc.org>
>Subject: What is it???
>
>Goodmorning!
>
>We saw something today that we have not seen before. We had a colon with an
>incredible black pigment in the sub-mucosa. It is very black and abundant.
>Reminds me of carbon in the lung. Does not stain with Iron stain. Any ideas?
>
>Marg
>
>Marjorie Hagerty H.T. (ASCP) H.T.L., Q IHC
>Supervisor, Anatomic Pathology
>Eisenhower Medical Center
>39-000 Bob Hope Drive
>Rancho Mirage, CA 92270
>Marg
>
ounds like it could be Melanosis coli.The pigment is autofluorescent,
weakly PAS positive and weakly sudanophilic.Found mainly in macrophages in
the lamina propria , it is assosciated with prolonged use of purgatives eg
cascara also linked with chronic obstruction.
Allan.
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 02:29:27 -0600
>From: David Taylor Manager <DTMan@KINGMOWER.COM.AU>
>Subject: RE: Alcohol Exposure
>
>
>Most histo-techs I know moniter their own exposure to alcohol. Coming from
>Adelaide, South Australia ( just next door to the Barossa Valley), it's
>usually red wine but beer is OK too. David
>
>David Taylor
>Laboratory Manager
>Drs King & Mower
>Adelaide, Australia
>
>- -----Original Message-----
>From: Mplhisto@aol.com [mailto:Mplhisto@aol.com]
>Sent: Friday, 16 March 2001 12:38
>To: histonet@pathology.swmed.edu
>Subject: Alcohol Exposure
>
>
> Does anyone know if or where we could monitor Alcohol exposure.
>
>
>
>
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><DIV><FONT face=Arial color=#0000ff size=2><SPAN
class=659580207-16032001>Most
>
>histo-techs I know moniter their own exposure to alcohol. Coming from
>Adelaide,
>South Australia ( just next door to the Barossa Valley), it's usually red
wine
>
>but beer is OK too. David
><P><FONT face=Arial size=2>David Taylor</FONT> <BR><FONT face=Arial
>size=2>Laboratory Manager</FONT> <BR><FONT face=Arial size=2>Drs King &
>Mower</FONT> <BR><FONT face=Arial size=2>Adelaide, Australia</FONT>
></P></SPAN></FONT></DIV>
><BLOCKQUOTE>
> <DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
> size=2>-----Original Message-----<BR><B>From:</B> Mplhisto@aol.com
> [mailto:Mplhisto@aol.com]<BR><B>Sent:</B> Friday, 16 March 2001
> 12:38<BR><B>To:</B> histonet@pathology.swmed.edu<BR><B>Subject:</B>
Alcohol
> Exposure<BR><BR></FONT></DIV><FONT face=arial,helvetica><FONT
color=#000040
> size=2> Does anyone know if or where we could monitor Alcohol
> exposure.</FONT> </FONT></BLOCKQUOTE></BODY></HTML>
>
>- ------_=_NextPart_001_01C0ADE8.9B9030F0--
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 03:29:17 -0600
>From: Mark Hammond <mjh80@cam.ac.uk>
>Subject: Re: Alcohol Exposure
>
>Dear Histonet,
>
>Usually when I can no longer stand up - my exposure level is at optimum.
>
>Mark
>
>Mplhisto@aol.com wrote:
>
>> Does anyone know if or where we could monitor Alcohol exposure.
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 07:40:49 -0600
>From: Scott MacDonald <macdonald@qmpls.org>
>Subject: Stain Criteria or Checklists
>
>Does anyone have or know where I can obtain checklists or lists of criteria
>for evaluating H&E and special stains (not immunohistochemical stains)?
>Thanks,
>Scott
>
>Scott MacDonald
>Technological Consultant
>Quality Management Program - Laboratory Services (QMP-LS)
>501-250 Bloor Street East
>Toronto, Ontario M4W 1E6
>
>Phone: (416) 323-9540, ext. 222
>Toll Free: 1-877-323-9540, ext. 222
>Fax: (416) 323-9324
>E-mail: macdonald@qmpls.org
>www.qmpls.org
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 09:07:03 -0600
>From: "Barry Rittman" <brittman@mail.db.uth.tmc.edu>
>Subject: Re: Stain for unmineralized bone
>
>Laurie
>The stain tha you are refering to is Heidenhain's Azan stain. It is a
>beautiful strain with strong vibrant colors but often requires some fine
>tuning.
>While this is a stain that I would prefer, in truth, many of the
>trichromes including Mallory's trichrome can be used to distinguish
>osteoid from mineralized bone.
>The most important thing is that if you are carrying out measurements on
>the amount of osteoid that you use thin sections so that there is no
>penumbra effect.
>Barry
>
>
>Laurie Colbert wrote:
>
>> Does anyone know of a special stain for unmineralized bone (pre-bone
>> material)? Is there a stain called Azan(?) for this?
>>
>> Laurie Colbert
>> Huntington Memorial Hospital
>> Pasadena, CA
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 09:25:34 -0600
>From: AndreaH@imclone.com
>Subject: IHC on kidney -Reply
>
>
>I would avoid using a biotin system at all on kidney or other biotin rich
>tissues. Make your life easier. For example, DAKO's Envision systems is
>really good if you are using mouse or rabbit antibodies. Otherwise you
>could directly HRP labeled secondaries or primaries.
>
>Andrea
>
>>----------------------------------------------------------------------
>>
>>Date: 12 Mar 2001 15:32:21 -0600
>>From: Tony Henwood <AnthonyH@chw.edu.au>
>>Subject: IHC on kidney -Reply
>>
>>Katie,
>>
>>Beware kidney is rich in biotin, so blocking is probably a good idea. AND
>>keep a careful eye on your negative controls.
>>
>>Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
>>Laboratory Manager
>>The Children's Hospital at Westmead,
>>Locked Bag 4001, Westmead, 2145, AUSTRALIA.
>>Tel: (02) 9845 3306
>>Fax: (02) 9845 3318
>>
>>>>> "Bennett, Catherine (Katie)" <cbennett@lrri.org> 13/March/2001
>>04:46am >>>
>>I have never done IHC staining on kidney tissue before and was
>>wondering if
>>an avidin/biotin blocking step is needed and/or useful.
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 09:32:42 -0600
>From: Cathy Mayton <Cathy@wasatchhisto.com>
>Subject: fixing tet labeled bone
>
>There are a couple ways to fix tet labeled bone. Either fix initially in
>10% neutral buffered formalin or go right into 70% ethanol. I believe some
>techs even use a product called Pen-Fix. I co-authored a paper that may be
>helpful to your study: Sanderson-Mayton, C, Clark, N., Bachus, K. N.: A
>new look at formalin fixation for fluorochrome labeled bone. J of
>Histotechnol, 21(4)299-302,1998.
>
>Good luck with your study and I hope this helps.
>Regards,
>Cathy
>
>
>*********************************
>GLP Compliant Laboratory
>
>Cathy A. Mayton
>Project Director
>Wasatch Histo Consultants, Inc.
>www.wasatchhisto.com
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 09:39:10 -0600
>From: Cathy Mayton <Cathy@wasatchhisto.com>
>Subject: decal and fluorochrome labels
>
>Yes, you will lose all your labels if the bone is decalcified. If you want
>to preserve the labels then the bone must be left mineralized, embedded and
>sectioned either microtome or ground sections.
>
>Good luck,
>Regards,
>Cathy
>
>
>
>*********************************
>GLP Compliant Laboratory
>
>Cathy A. Mayton
>Project Director
>Wasatch Histo Consultants, Inc.
>www.wasatchhisto.com
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 09:48:55 -0600
>From: Cathy Mayton <Cathy@wasatchhisto.com>
>Subject: bone stain for non-mineralized/mineralized tissues
>
>I am not familiar with the stain you are searching for but you may want to
>try an additional stain called Sanderson's Rapid Bone Stain, which is sold
>by Surgipath Medical. The stain clearly differentiates between mineralized
>and non-mineralized bone. It also defines dead bone. You may want to
>refer to the following paper: Sanderson, C., Bachus, K. N., Staining
>technique to differentiate mineralized and demineralized bone in ground
>sections. J Histotechnol, 20(2)119-122,1997.
>
>Hope this helps,
>Cathy
>
>
>*********************************
>GLP Compliant Laboratory
>
>Cathy A. Mayton
>Project Director
>Wasatch Histo Consultants, Inc.
>www.wasatchhisto.com
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 09:49:13 -0600
>From: MontagueDonnaC@uams.edu
>Subject: RE: Alcohol Exposure
>
>After a particularly vexing day I fancy a good single malt actually.
>Regards, Donna Montague, UAMS Orthopaedic Reseach, Little Rock, AR,USA
>
>- -----Original Message-----
>From: David Taylor Manager [mailto:DTMan@KINGMOWER.COM.AU]
>Sent: Friday, March 16, 2001 1:14 AM
>To: Histonet (E-mail)
>Subject: RE: Alcohol Exposure
>
>
>Most histo-techs I know moniter their own exposure to alcohol. Coming from
>Adelaide, South Australia ( just next door to the Barossa Valley), it's
>usually red wine but beer is OK too. David
>
>David Taylor
>Laboratory Manager
>Drs King & Mower
>Adelaide, Australia
>
>- -----Original Message-----
>From: Mplhisto@aol.com [mailto:Mplhisto@aol.com]
>Sent: Friday, 16 March 2001 12:38
>To: histonet@pathology.swmed.edu
>Subject: Alcohol Exposure
>
>
> Does anyone know if or where we could monitor Alcohol exposure.
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 11:27:29 -0600
>From: "Brennan, Liam" <Liam.Brennan@bll.n-i.nhs.uk>
>Subject: FW: Stain Criteria or Checklists
>
>Try the Uk's Technical Quality assurance for Histopathology. Their Website
>is www.hteqa.demon.co.uk (I think!!).
> -Liam
>
>> ----------
>> From: Scott MacDonald[SMTP:macdonald@qmpls.org]
>> Sent: 16 March 2001 13:14
>> To: 'histonet@pathology.swmed.edu'
>> Subject: Stain Criteria or Checklists
>>
>> Does anyone have or know where I can obtain checklists or lists of
>> criteria
>> for evaluating H&E and special stains (not immunohistochemical stains)?
>> Thanks,
>> Scott
>>
>> Scott MacDonald
>> Technological Consultant
>> Quality Management Program - Laboratory Services (QMP-LS)
>> 501-250 Bloor Street East
>> Toronto, Ontario M4W 1E6
>>
>> Phone: (416) 323-9540, ext. 222
>> Toll Free: 1-877-323-9540, ext. 222
>> Fax: (416) 323-9324
>> E-mail: macdonald@qmpls.org
>> www.qmpls.org
>>
>>
>>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 11:27:49 -0600
>From: "Dr. Allen A. Smith" <asmith@mail.barry.edu>
>Subject: hematoxylin crystals
>
>Hematoxylin crystals haven't been on the market for years. The powder works
>fine for Ehrlich's and for every other variant that I've tried (about 20).
>If you put the powder and the ethanol into a small flask with a magnetic
>stirring bug and let it stir for a while, all of a good quality powder will
>dissolve.
> Allen A. Smith
> School of Graduate Medical Sciences
> Podiatric Medicine and Surgery
> Barry University
> Miami Shores, Florida
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 11:28:28 -0600
>From: "Connolly, Brett M" <brett_connolly@merck.com>
>Subject: RE: Alcohol Exposure
>
>
>What a timely queshtion. I also shelf monitor, ie. last night whilst
>preparing for St. Pat's Day I went to brush shomething off my shoulder.....
>when I discovered I was trying to brush floor from my shoulder I knew I
>exsheeded my exshposure level.
>Shtill recovering.
>
>Slainte !!
>
>Brett
>
>Brett M. Connolly, Ph.D.
>Merck Research Laboratories
>Dept. of Pharmacology
>WP26A-3000
>PO Box 4
>West Point, PA 19486
>ph. 215-652-2501
>fax. 215-652-2075
>email. brett_connolly@merck.com
>
>- -----Original Message-----
>From: Mplhisto@aol.com [mailto:Mplhisto@aol.com]
>Sent: Thursday, March 15, 2001 9:08 PM
>To: histonet@pathology.swmed.edu
>Subject: Alcohol Exposure
>
>
> Does anyone know if or where we could monitor Alcohol exposure.
>
>
>
>******************* NOTE *******************
>There may be important message content
>contained in the following MIME Information.
>********************************************
>
>
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><DIV><FONT color=#0000ff face=Arial size=2><SPAN
>class=781040015-16032001>What a timely queshtion. I also shelf
>monitor, ie. last night whilst preparing for St. Pat's Day I went to brush
>shomething off my shoulder..... when I discovered I was trying to brush
floor
>from my shoulder I knew I exsheeded my exshposure level. </SPAN></FONT></DIV>
><DIV><FONT color=#0000ff face=Arial size=2><SPAN
>class=781040015-16032001>Shtill
>recovering.</SPAN></FONT></DIV>
><DIV><FONT color=#0000ff face=Arial size=2><SPAN
>class=781040015-16032001></SPAN></FONT> </DIV>
><DIV><FONT face=Arial><SPAN class=781040015-16032001><FONT
>face="Times New Roman MT Extra Bold">
><P><FONT size=2><FONT color=#0000ff>Sláinte<FONT face=Arial><SPAN
>class=081184016-16032001> !! </SPAN></FONT></FONT></FONT></P></FO
NT></SPAN></FONT></DIV>
><DIV><FONT color=#0000ff face=Arial size=2><SPAN
>class=781040015-16032001>Brett
></SPAN></FONT></DIV>
><DIV><FONT color=#0000ff face=Arial size=2><SPAN
>class=781040015-16032001></SPAN></FONT> </DIV>
><DIV><FONT color=#0000ff face=Arial size=2><SPAN class=781040015-16032001>
><P><FONT face=Arial size=2>Brett M. Connolly, Ph.D.</FONT> <BR><FONT
>face=Arial
>size=2>Merck Research Laboratories</FONT> <BR><FONT face=Arial
size=2>Dept. of
>
>Pharmacology</FONT> <BR><FONT face=Arial size=2>WP26A-3000</FONT> <BR><FONT
>face=Arial size=2>PO Box 4</FONT> <BR><FONT face=Arial size=2>West Point, PA
>19486</FONT> <BR><FONT face=Arial size=2>ph. 215-652-2501</FONT> <BR><FONT
>face=Arial size=2>fax. 215-652-2075</FONT> <BR><FONT face=Arial
size=2>email.
>brett_connolly@merck.com</FONT> </P></SPAN></FONT></DIV>
><DIV align=left class=OutlookMessageHeader dir=ltr><FONT face=Tahoma
>size=2>-----Original Message-----<BR><B>From:</B> Mplhisto@aol.com
>[mailto:Mplhisto@aol.com]<BR><B>Sent:</B> Thursday, March 15, 2001 9:08
>PM<BR><B>To:</B> histonet@pathology.swmed.edu<BR><B>Subject:</B> Alcohol
>Exposure<BR><BR></FONT></DIV><FONT face=arial,helvetica><FONT color=#000040
>size=2> Does anyone know if or where we could monitor Alcohol
>exposure.</FONT> </FONT></BODY></HTML>
>
>- --Boundary_(ID_lRpF9rHBZuhCz9nTZDcs3w)--
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 13:08:25 -0600
>From: "LEROY BROWN" <lhbhcs@pioneernet.net>
>Subject: re:gms/pas
>
>Dear Histonet,
>
>Does anyone have a method for doing a combination gms/pas stain for paraffin
>sections?
>
>Thanks
>
>LeRoy Brown HT(ASCP) HTL
>Histology Consultation Services
>P.O. Box 1109
>85 SE 8th Ave.
>Oak Harbor, WA 98277
>****
>1-360-675-9173
>fax 1-360-675-5820
>web site www.histocs.com
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 13:09:09 -0600
>From: "Hagerty, Marjorie A." <mhagerty@emc.org>
>Subject: What is it???
>
>Goodmorning!
>
>We saw something today that we have not seen before. We had a colon with an
>incredible black pigment in the sub-mucosa. It is very black and abundant.
>Reminds me of carbon in the lung. Does not stain with Iron stain. Any ideas?
>
>Marg
>
>Marjorie Hagerty H.T. (ASCP) H.T.L., Q IHC
>Supervisor, Anatomic Pathology
>Eisenhower Medical Center
>39-000 Bob Hope Drive
>Rancho Mirage, CA 92270
>Marg
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 13:26:54 -0600
>From: conmac@cc.usu.edu
>Subject: The Irish in me
>
>
>
>I heard St. Patrick's day celebrations have been canceled in most parts
>of ireland due to the Hoof & Mouth outbreak. If this is true, I'm so
>sorry to hear this. I hope to brighten your day by wishing all Irish
>Histonetters a most pleasant and happy day, anyway!
>
>and to all the rest of you... Let the party begin!! *G*
>
>HAPPY ST. PATRICK'S DAY!!!
>
>Connie McManus (whose good Irish name comes by way of marriage)
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 13:27:10 -0600
>From: "Dr. Allen A. Smith" <asmith@mail.barry.edu>
>Subject: Re: LACMOID RE: OOCYTES - HELP!!!!
>
>You can get fresh lacmoid (cat. no. 27,472-0, $23.60 for 10 g.) from Aldrich
>(1-800-558-9160).
>Lacmoid is a bis-resorcinyl oxazine. Orcein is a mixture of mono-resorcinyl
>oxazines.
>
> Allen A. Smith
> Barry University
> Miami Shores, Florida
>
>- ----- Original Message -----
>From: Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au>
>To: <histoNet@Pathology.swmed.edu>
>Sent: Thursday, March 15, 2001 12:05 AM
>Subject: LACMOID RE: OOCYTES - HELP!!!!
>
>
>>
>> Dear Histonetters,
>>
>> Can anyone give me any suggestions on how to prepare a lacmoid solution
>for ooctye staining?
>>
>> We found it very difficult to get lacmoid crystals, and the batch we
>finally got our hands on is very old. I needed to prepare a 1% lacmoid
>solution and placed 1g of lacmoid crystals in 45% acetic acid and left it on
>a stirrer/hot plate for 2 hours at a moderate heat. When I filtered it
>there was still a large amount of crystal undissolved (about half), yet a
>reasonable amount of colour in the solution. The undissolved crystal was
>more grey in colour than they grey/burgundy of the original crystal.
>>
>> Does anyone know if this is an appropriate method of preparation? I can't
>believe my solution is a 1% solution if there is undissolved crystal?
>>
>>
>> BRUCE ABALOZ
>> HISTOLOGIST
>> DEPARTMENT of ZOOLOGY * PH: +61 3 83446282
>> The UNIVERSITY of MELBOURNE * FAX: +61 3 83447909
>> Parkville, Victoria.3010 * EMAIL:
>b.abaloz@zoology.unimelb.edu.au
>> AUSTRALIA.
>> DANCE, LIKE NO - ONE'S WATCHING!!
>> YOU ARE A SPIRITUAL BEING HAVING A HUMAN EXPERIENCE!!
>>
>>
>This message is confidential;its contents do not constitute a commitment by
>The University Of Melbourne except where provided for in a written agreement
>between you and The University Of Melbourne. Any unauthorised disclosure,
>use or dissemination, either whole or partial, is prohibited. If you are not
>the intended recipient of the message, please notify the sender immediately.
>>
>>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 15:01:18 -0600
>From: "P. Emry" <emry@u.washington.edu>
>Subject: RE: Alcohol Exposure
>
>I recently spent $106 on a prescription given to me in the Pain Clinic. I
>couldn't remember my name and nearly set the lab on fire leaving a
>hotplate on all night. After 3 martinis, shaken not stirred, I found the
>relief I sought, could still function and found the world a much happier
>place...everybodies purdy....alcohol exposure...I highly recommend it.
>
>Trisha
>
>
>On Fri, 16 Mar 2001, Connolly, Brett M wrote:
>
>> What a timely queshtion. I also shelf monitor, ie. last night whilst
>> preparing for St. Pat's Day I went to brush shomething off my shoulder.....
>> when I discovered I was trying to brush floor from my shoulder I knew I
>> exsheeded my exshposure level.
>> Shtill recovering.
>>
>> Slainte !!
>>
>> Brett
>>
>> Brett M. Connolly, Ph.D.
>> Merck Research Laboratories
>> Dept. of Pharmacology
>> WP26A-3000
>> PO Box 4
>> West Point, PA 19486
>> ph. 215-652-2501
>> fax. 215-652-2075
>> email. brett_connolly@merck.com
>>
>> -----Original Message-----
>> From: Mplhisto@aol.com [mailto:Mplhisto@aol.com]
>> Sent: Thursday, March 15, 2001 9:08 PM
>> To: histonet@pathology.swmed.edu
>> Subject: Alcohol Exposure
>>
>>
>> Does anyone know if or where we could monitor Alcohol exposure.
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 15:28:40 -0600
>From: "Laboratory" <hymclab@newnorth.net>
>Subject: Re: Meditech "X" number
>
>We also get alot off "drop off" (outreach) specimens. What we've done to
>ensure good TAT is to use the short "REF" admission (one page) and use a
>dummy client in the client field. Then we send the face sheet of the
>admission we've created along with the insurance info to admissions and they
>go through a routine that changes the short client admission into a long
>admission. This seems to make both departments happy----as you know we are
>not admissions staff and they don't understand turn around time.
>Feel free to contact me if you need any more info.
>
>Thanks-
>Dawn
>Howard Young Medical Center
>Woodruff, WI
>(715)356-8174
>
>- -----Original Message-----
>From: CMD1352@aol.com <CMD1352@aol.com>
>To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
>Date: Thursday, March 15, 2001 10:21 PM
>Subject: Meditech "X" number
>
>
>>If you are a Meditech user, please read on:
>>The majority of our work are outpatient specimen drop offs. Each
>requisition
>>must be given an "X" number before we can process the specimen. The
>>Admitting process is 7-8 pages long. Unfortunately our Admitting
>department
>>cannot handle the overload, therefore, our turn around time suffers. Some
>>requisitions do not return to the lab for 4-5 hours. How do Meditech users
>>handle specimen drop off (SDO) requisitions? Does any lab admit their own
>>outpatient reqs on a short form, then enter the rest of the admitting
>>demographics at a later time? Please advise me on an easier and faster
>way
>>to admit these requisitions and please be specific. Thank you.
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 15:29:05 -0600
>From: "Anne Sanders" <brsales@brinstrument.com>
>Subject: HistoNet archives
>
>I need to access the HistoNet archives. The link listed on the Histotech's
>Portal homepage is no longer a good link; can someone point me in the right
>direction?!
>Thank you!
>Anne Sanders
>B/R Instrument Corp.
>www.brinstrument.com
>brsales@brinstrument.com
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 15:53:08 -0600
>From: "P. Emry" <emry@u.washington.edu>
>Subject: hoof and mouth
>
>I heard an interview on npr yesterday with a woman who's heard was
>infected and destroyed in Wales. Made me cry, the description of the
>cattle and her loss.
>
>Does anyone out there know what the disease is and why after so many
>years of being around there has not been a successful treatment or
>inoculation developed?
>
>Thanks,
>
>Trisha
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 15:53:47 -0600
>From: "LEROY BROWN" <lhbhcs@pioneernet.net>
>Subject: Re: re:gms/pas
>
>Dear Petty,
> Thanks for your input. I will pass along any answers to the question of
>gms/pas stains that come my way.
>
>LeRoy Brown HT(ASCP) HTL
>Histology Consultation Services
>P.O. Box 1109
>85 SE 8th Ave.
>Oak Harbor, WA 98277
>****
>1-360-675-9173
>fax 1-360-675-5820
>web site www.histocs.com
>
>- ----- Original Message -----
>From: "Lee & Peggy Wenk" <lpwenk@mail.netquest.com>
>To: "LEROY BROWN" <lhbhcs@pioneernet.net>
>Sent: Friday, March 16, 2001 12:56 PM
>Subject: Re: re:gms/pas
>
>
>> Hmmm. Interesting concept. Never tried it or thought about it before.
>>
>> Let's try to think through some theory and logic.
>>
>> In the GMS, the silver attaches to aldehydes that you created on the
>> fungus via the periodic acid or chromic acid, thus staining the fungus
>> black.
>>
>> On the PAS, the Schiff attaches to the aldehydes that you created on
>> the fungus via periodic acid, staining the fungus magenta.
>>
>> It seems that, if you attach the silver to the aldehydes first, there
>> wouldn't be
>> any aldehydes left for the Schiff to attach to. And the same with first
>> attaching
>> the Schiffs and then trying to attach the silver - no leftover aldehydes
>for
>> the second stain.
>>
>> However, there are could be enough aldehydes on the fungus that
>> didn't pick up the first chemical, so would be available to pick up
>> the second chemical. But black-magenta fungus? Isn't that overkill?
>>
>> Just theorizing, I could possibly see a use for this in trying to stain
>> fungus
>> and Pneumocystis carinii (PC) on the same section. In that case, stain
>> with PAS first (which would demonstrate the fungus, but NOT the PC).
>> Then, without going into chromic acid or periodic acid a second time
>> (don't need to oxidize the fungus as you already did that with the PAS),
>> go into the methenamine silver to demonstrate the PC.
>>
>> I'd love to hear if anyone does this, why, and how it looks. Always
>> looking for new things to learn.
>>
>> Peggy A. Wenk, HTL(ASCP)
>> William Beaumont Hospital
>> Royal Oak, MI
>>
>>
>>
>> ----- Original Message -----
>> From: "LEROY BROWN" <lhbhcs@pioneernet.net>
>> To: "histonet" <histonet@pathology.swmed.edu>
>> Sent: Friday, March 16, 2001 12:57 PM
>> Subject: re:gms/pas
>>
>>
>> > Dear Histonet,
>> >
>> > Does anyone have a method for doing a combination gms/pas stain for
>> paraffin
>> > sections?
>> >
>> > Thanks
>> >
>> > LeRoy Brown HT(ASCP) HTL
>> > Histology Consultation Services
>> > P.O. Box 1109
>> > 85 SE 8th Ave.
>> > Oak Harbor, WA 98277
>> > ****
>> > 1-360-675-9173
>> > fax 1-360-675-5820
>> > web site www.histocs.com
>> >
>> >
>> >
>> >
>>
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 16:35:10 -0600
>From: "Fuller, Michelle" <mfuller@acmlab.com>
>Subject: RE: What is it???
>
>Hi-
>Our Pathologists saw something similar to this last week with two
>different cases. One case was a small bowel and the other one was a
>breast specimen. They are also interested to see if anyone has any
>ideas.
>
>Thanks, Michelle
>
>Michelle Fuller
>Histology Supervisor
>ACM Medical Laboratory, Inc.
>Rochester, NY 14624
>
>- -----Original Message-----
>From: Hagerty, Marjorie A. [mailto:mhagerty@emc.org]
>Sent: Friday, March 16, 2001 1:37 PM
>To: 'Histonet'
>Subject: What is it???
>
>
>Goodmorning!
>
>We saw something today that we have not seen before. We had a colon with
>an
>incredible black pigment in the sub-mucosa. It is very black and
>abundant.
>Reminds me of carbon in the lung. Does not stain with Iron stain. Any
>ideas?
>
>Marg
>
>Marjorie Hagerty H.T. (ASCP) H.T.L., Q IHC
>Supervisor, Anatomic Pathology
>Eisenhower Medical Center
>39-000 Bob Hope Drive
>Rancho Mirage, CA 92270
>Marg
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 17:54:28 -0600
>From: RSRICHMOND@aol.com
>Subject: What is it??? -black pigment in colon
>
>Marjorie Hagerty H.T. (ASCP) H.T.L., Q IHC at the Eisenhower Medical Center,
>Rancho Mirage, California asks:
>
>
>>>We saw something today that we have not seen before. We had a colon with
an
>incredible black pigment in the sub-mucosa. It is very black and abundant.
>Reminds me of carbon in the lung. Does not stain with Iron stain. Any
ideas?<<
>
>This spectacular blackening of the mucosal surface of the colon is called
>melanosis coli. It's caused by chronic ingestion of emodin (anthraquinone)
>cathartics such as senna, cascara sagrada, and aloe vera. (Some say the
lowly
>prune contains a bit of emodin also.)
>
>The pigment, supposedly arising in injured mitochondria, is found in
>macrophages in the lamina propria of the mucosa. It looks like melanin and
to
>some degree stains like melanin, though on electron microscopy it clearly
>isn't melanin. Thus it stains with silver reducing (argyrophil) techniques
>such as Fontana- Masson, but does not stain as hemosiderin with the Perls
>prussian blue technique. It usually isn't acid-fast.
>
>Only the colon is affected. Melanosis stops abruptly at the ileocecal valve.
>Sometimes the lymphoid nodules stand out like little white stars in a black
>sky. The obstructing cancer that causes the patient to take the laxative
>isn't colored either.
>
>Emodin cathartics should be banned from commerce, but they're very popular
>with the herbal medicine people and are common ingredients of quack weight
>reducing formulas. Having a black colon doesn't do you any harm, but the
>cathartic eventually destroys the nerve cells of the myenteric plexus and
>then you REALLY get constipated.
>
>Bob Richmond
>Samurai Pathologist
>Knoxville TN
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 18:58:19 -0600
>From: "Steve Machin UK" <stevemachinuk@yahoo.co.uk>
>Subject: Re: hoof and mouth
>
>
>- --- "P. Emry" <emry@u.washington.edu> wrote: > I heard
>an interview on npr yesterday with a woman
>> who's heard was
>> infected and destroyed in Wales. Made me cry, the
>> description of the
>> cattle and her loss.
>>
>> Does anyone out there know what the disease is and
>> why after so many
>> years of being around there has not been a
>> successful treatment or
>> inoculation developed?
>>
>> Thanks,
>>
>> Trisha
>>
>>
>>
>>From Steve Machin in Derbyshire UK, an area where all
>footpaths into the countyside are bocked by hazard
>tape and signs warning of five thousand pound fines
>for entering.
>
>I understand that the disease is viral, self limiting
>and the animals recover. Livestock can be vaccinated
>against it and the disease isn't harmful to humans.
>So I'm sure you are wondering which it's a problem so
>serious to slaughter entire herds even if their only
>crime is to be within miles of an infected animal. I
>dont know the answer to that, perhaps you should ask
>the "Ministry for Agriculture Foods and Fisheries". I
>suspect it is to do with "the value of exports".
>p.s. I am not a veggie but I'm thinking about it.
>
>
>____________________________________________________________
>Do You Yahoo!?
>Get your free @yahoo.co.uk address at http://mail.yahoo.co.uk
>or your free @yahoo.ie address at http://mail.yahoo.ie
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 19:17:48 -0600
>From: "Hagerty, Marjorie A." <mhagerty@emc.org>
>Subject: RE: What is it??? -black pigment in colon
>
>Samurai,
>
>This pigment is in the submucosa not the lamina propria?
>
>Marg
>
>
>- -----Original Message-----
>From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com]
>Sent: Friday, March 16, 2001 2:23 PM
>To: histonet@pathology.swmed.edu
>Subject: What is it??? -black pigment in colon
>
>
>Marjorie Hagerty H.T. (ASCP) H.T.L., Q IHC at the Eisenhower Medical Center,
>
>Rancho Mirage, California asks:
>
>
>>>We saw something today that we have not seen before. We had a colon with
>an
>incredible black pigment in the sub-mucosa. It is very black and abundant.
>Reminds me of carbon in the lung. Does not stain with Iron stain. Any
>ideas?<<
>
>This spectacular blackening of the mucosal surface of the colon is called
>melanosis coli. It's caused by chronic ingestion of emodin (anthraquinone)
>cathartics such as senna, cascara sagrada, and aloe vera. (Some say the
>lowly
>prune contains a bit of emodin also.)
>
>The pigment, supposedly arising in injured mitochondria, is found in
>macrophages in the lamina propria of the mucosa. It looks like melanin and
>to
>some degree stains like melanin, though on electron microscopy it clearly
>isn't melanin. Thus it stains with silver reducing (argyrophil) techniques
>such as Fontana- Masson, but does not stain as hemosiderin with the Perls
>prussian blue technique. It usually isn't acid-fast.
>
>Only the colon is affected. Melanosis stops abruptly at the ileocecal valve.
>
>Sometimes the lymphoid nodules stand out like little white stars in a black
>sky. The obstructing cancer that causes the patient to take the laxative
>isn't colored either.
>
>Emodin cathartics should be banned from commerce, but they're very popular
>with the herbal medicine people and are common ingredients of quack weight
>reducing formulas. Having a black colon doesn't do you any harm, but the
>cathartic eventually destroys the nerve cells of the myenteric plexus and
>then you REALLY get constipated.
>
>Bob Richmond
>Samurai Pathologist
>Knoxville TN
>
>
>----------------------------------------------------------------------
>
>Date: 16 Mar 2001 20:19:01 -0600
>From: DDittus787@aol.com
>Subject: Re: Re - Automated staining machines
>
>
>Dear Ian
>I have the Cytologix Artisan, and the Ventana Nexes, both are good, timing
>for an an IHC and specials run on the Artisan vary according to number of
>slides and which stains , they have some stains that are 8 min some 1 hr,
it
>is dependent on your needs and volume. The stains are gorgeous. The nexes
>quality is good also, however specials and IHC are run on seperate modules,
>some specials must be run alone, so you may have to wait to do others.
> Dana
>
>
>
>******************* NOTE *******************
>There may be important message content
>contained in the following MIME Information.
>********************************************
>
>
>- ------------------ MIME Information follows ------------------
>
>
>- --part1_8c.3cdc2b1.27e4154b_boundary
>Content-Type: text/plain; charset="US-ASCII"
>Content-Transfer-Encoding: 7bit
>
><<<<<< See above "Message Body" >>>>>>
>
>- --part1_8c.3cdc2b1.27e4154b_boundary
>Content-Type: text/html; charset="US-ASCII"
>Content-Transfer-Encoding: 7bit
>
><HTML><FONT FACE=arial,helvetica><FONT SIZE=2>Dear Ian
><BR>I have the Cytologix Artisan, and the Ventana Nexes, both are good,
timing
>
><BR>for an an IHC and specials run on the Artisan vary according to number
of
><BR>slides and which stains , they have some stains that are 8 min some
>1 hr, it
><BR>is dependent on your needs and volume. The stains are gorgeous. The
nexes
><BR>quality is good also, however specials and IHC are run on seperate
>modules,
><BR>some specials must be run alone, so you may have to wait to do
>others.
><BR> Dana</FONT></HTML>
>
>- --part1_8c.3cdc2b1.27e4154b_boundary--
>
>
>Here are the messages received yesterday!
>
>
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