Re: Anti-SMA in mouse

From:antje.marcantonio@pharma.Novartis.com


Hello Andrea,

we are using anti-sma clone 1A4 from Dako, 1:100 without pretreatment on
"optimal" fixed mouse tissue. Overfixed tissue we treat with trypsin .
Then we use the ARK Kit from Dako , because of the mouse primary ab on
mouse tissue problem. We have absolut NO background.
Feel free to contact me if you have more questions.

Antje Marcantonio
Novartis Pharma AG, Basel/Switzerland




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